No, you can't. LOT number must not be duplicated in your data.
In-line with the Traceability standard there is always only one set of Traceability information per LOT. As a result, this information set will always be the record that you submitted last, overwriting the others.
Combination of GTIN and LOT number should be unique. Together with the LOT number and GTIN, you will be able to build the unique identifier.
Combination A: for instance 2 different articles are coming from the same LOT:
Salmon fillet - Lot number: 06082020; GTIN: 01020304050607
Wild Salmon fillet - Lot number: 06082020; GTIN: 02030405060708
Combination B: for instance 2 same articles are coming from different LOTs:
Salmon fillet - Lot number: 06082020; GTIN: 01020304050607
Salmon fillet - Lot number: 05082020; GTIN: 01020304050607
In other words, if Traceability information is different and can be separated (e.g. Farm address, slaughtering date), different LOTs should be also given.
Please refer also to the article What is a LOT number?